A review on restriction fragment length polymorphism: usefulness for characterization of Escherichia coli
Keywords:PCR-RFLP, heterozygous, homozygous, Escherichia coli
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method is a technique to detect the polymorphisms, especially single nucleotide polymorphisms (SNPs) in the genome, using restriction enzymes. One of the most important applications of PCR-RFLP is the detection of mutations. In the PCR-RFLP method, first the mutated fragment (also non-mutated fragment) is amplified by the polymerase chain reaction using two specific primers designed for this purpose and after enzymatic digestion, electrophoresis is done. In a part of this review, identification of genotypes by PCR-RFLP has been interpreted. The capacity of PCR-RFLP technique to identify heterozygous individuals from homozygous ones makes it a powerful method in the study of phylogenetic relationships. In sum, in the field of molecular biology, using PCR-RFLP technique has some advantages. The technique is a genetic method and not influenced by environmental factors. The method is co-dominant that heterozygous genotype is detectable in it. The method has high repeatability and is a low cost method that needs no high technology. The technique can be optimized quickly and the analysis of results is easy. I the second part of this study, PCR-RFLP usefulness for molecular characterization of Escherichia coli has been reviewed.